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Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells

dc.contributor.authorKilgas, Susan
dc.contributor.authorKiltie, Anne E.
dc.contributor.authorRamadan, Kristijan
dc.date.accessioned2021-12-07T15:36:01Z
dc.date.available2021-12-07T15:36:01Z
dc.date.issued2021-12-17
dc.identifier.citationKilgas , S , Kiltie , A E & Ramadan , K 2021 , ' Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells ' , STAR Protocols , vol. 2 , no. 4 , 100978 . https://doi.org/10.1016/j.xpro.2021.100978en
dc.identifier.issn2666-1667
dc.identifier.otherPURE: 209764062
dc.identifier.otherPURE UUID: bbec5f92-065d-4cb5-b96d-0f1552e1ea37
dc.identifier.otherScopus: 85119911953
dc.identifier.otherORCID: /0000-0001-7208-2912/work/104426466
dc.identifier.urihttps://hdl.handle.net/2164/17639
dc.descriptionAcknowledgments This work was funded by the MRC Program grant MC_PC 12001/1 and MC_UU_00001/1 to K.R., and S.K. was supported by the MRC Oxford Institute of Radiation Oncology (OIRO) Cancer Research UK (CRUK) Studentship. We thank Dr. Rhodri Wilson from the microscopy imaging core (University of Oxford, Department of Oncology) for his technical advice and assistance. Graphical abstract was created with BioRender.en
dc.format.extent15
dc.language.isoeng
dc.relation.ispartofSTAR Protocolsen
dc.rightsThis is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectAntibodyen
dc.subjectCell Biologyen
dc.subjectCell-based Assaysen
dc.subjectMicroscopyen
dc.subjectMolecular Biologyen
dc.subjectBiochemistry, Genetics and Molecular Biology(all)en
dc.subjectNeuroscience(all)en
dc.subjectImmunology and Microbiology(all)en
dc.subjectMedical Research Council (MRC)en
dc.subjectMC_PC 12001/1en
dc.subjectMC_UU_00001/1en
dc.titleImmunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cellsen
dc.typeJournal articleen
dc.contributor.institutionUniversity of Aberdeen.Rowett Instituteen
dc.description.statusPeer revieweden
dc.description.versionPublisher PDFen
dc.identifier.doihttps://doi.org/10.1016/j.xpro.2021.100978
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85119911953&partnerID=8YFLogxKen
dc.identifier.vol2en
dc.identifier.iss4en


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