This is the peer reviewed version of the following article: Lorenz A (2015) New cassettes for single-step drug-resistance and prototrophic marker switching in fission yeast. – Yeast 32 (12): 703-710, which has been published in final form at [http://dx.doi.org/10.1002/yea.3097]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.