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Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region

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dc.contributor.authorBalasubramanian, Sandhya
dc.contributor.authorAndreani, Matteo
dc.contributor.authorAndrade, Júlia Goncalves
dc.contributor.authorSaha, Tannishtha
dc.contributor.authorSundaravinayagam, Devakumar
dc.contributor.authorGarzón, Javier
dc.contributor.authorZhang, Wenzhu
dc.contributor.authorPopp, Oliver
dc.contributor.authorHiraga, Shin-Ichiro
dc.contributor.authorRahjouei, Ali
dc.contributor.authorRosen, Daniel B
dc.contributor.authorMertins, Philipp
dc.contributor.authorChait, Brian T
dc.contributor.authorDonaldson, Anne D
dc.contributor.authorDi Virgilio, Michela
dc.contributor.institutionUniversity of Aberdeen.Institute of Medical Sciencesen
dc.contributor.institutionUniversity of Aberdeen.Medical Sciencesen
dc.contributor.institutionUniversity of Aberdeen.Molecular and Cellular Functionen
dc.date.accessioned2022-04-28T20:15:03Z
dc.date.available2022-04-28T20:15:03Z
dc.date.issued2022-04-13
dc.descriptionAcknowledgements We thank all members of the Di Virgilio lab for their feedback and discussion; V Delgado-Benito (Di Virgilio lab, MDC, Berlin) for her contribution to the project development; L Keller (Di Virgilio lab, MDC, Berlin) for support with cloning, mutagenesis, and mice genotyping; C Brischetto (Scheidereit Lab, MDC, Berlin) for assistance with confocal microscopy; Aberdeen Proteomics facility (University of Aberdeen) for the mass spec analysis of Aph-induced hRIF1 phosphorylation; and the MDC FACS Core Facility and Dr. HP Rahn for support with cell sorting. Aliquots of ATRi and ATMi were gener- ously provided by AG Henssen (MDC and ECRC, Berlin). Figures 1B and D, 2A, and 4C contain items created with BioRender.com. This work was supported by ERC grant 638897 (to MDV), the Helmholtz- Gemeinschaft Zukunftsthema 'Immunology and Inflammation' ZT-0027 (to MDV), P41 GM109824 and P41 GM103314 (to BTC), and Cancer Research UK awards C1445/A19059 and DRCPGM\100,013 (to ADD and SH).en
dc.description.statusPeer revieweden
dc.format.extent30
dc.format.extent2366410
dc.identifier215347725
dc.identifier0693baea-272b-4103-a72b-f66f25ffa663
dc.identifier35416772
dc.identifier85128131062
dc.identifier000806238400001
dc.identifier.citationBalasubramanian, S, Andreani, M, Andrade, J G, Saha, T, Sundaravinayagam, D, Garzón, J, Zhang, W, Popp, O, Hiraga, S-I, Rahjouei, A, Rosen, D B, Mertins, P, Chait, B T, Donaldson, A D & Di Virgilio, M 2022, 'Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region', eLife, vol. 11, e75047. https://doi.org/10.7554/eLife.75047en
dc.identifier.doi10.7554/eLife.75047
dc.identifier.otherPubMedCentral: PMC9007588
dc.identifier.otherORCID: /0000-0002-8722-3869/work/112495123
dc.identifier.otherORCID: /0000-0002-5093-8385/work/189637779
dc.identifier.urihttps://hdl.handle.net/2164/18493
dc.identifier.vol11en
dc.language.isoeng
dc.relation.ispartofeLifeen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subjectAnimalsen
dc.subjectDNA/metabolismen
dc.subjectDNA Breaks, Double-Strandeden
dc.subjectDNA Repairen
dc.subjectDNA Replicationen
dc.subjectMiceen
dc.subjectPhosphorylationen
dc.subjectTelomere-Binding Proteins/geneticsen
dc.subjectR Medicineen
dc.subjectEuropean Research Councilen
dc.subject638897en
dc.subjectCancer Research UKen
dc.subjectC1445/A19059en
dc.subjectDRCPGM\100en
dc.subjectDRCPGM\013en
dc.subjectOtheren
dc.subjectP41 GM109824en
dc.subjectP41 GM103314en
dc.subjectZT-0027en
dc.subject.lccRen
dc.titleProtection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered regionen
dc.typeJournal articleen

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