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Human gut Faecalibacterium prausnitzii deploy a highly efficient conserved system to cross-feed on β-mannan-derived oligosaccharides

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dc.contributor.authorLindstad, Lars J.
dc.contributor.authorLo, Galiana
dc.contributor.authorLeivers, Shaun
dc.contributor.authorLu, Zijia
dc.contributor.authorMichalak, Leszek
dc.contributor.authorPereira, Gabriel V.
dc.contributor.authorRøhr, Åsmund K.
dc.contributor.authorMartens, Eric C.
dc.contributor.authorMcKee, Lauren S.
dc.contributor.authorLouis, Petra
dc.contributor.authorDuncan, Sylvia H.
dc.contributor.authorWestereng, Bjørge
dc.contributor.authorPope, Phillip B.
dc.contributor.authorLa Rosa, Sabina Leanti
dc.contributor.institutionUniversity of Aberdeen.Medicine, Medical Sciences & Nutritionen
dc.contributor.institutionUniversity of Aberdeen.Rowett Instituteen
dc.date.accessioned2021-09-07T13:18:01Z
dc.date.available2021-09-07T13:18:01Z
dc.date.issued2021-06-01
dc.descriptionACKNOWLEDGMENTS We are grateful for support from The Research Council of Norway (FRIPRO program to P.B.P.: 250479; BIONÆR program to B.W.: 244259), the European Research Commission Starting Grant Fellowship (awarded to P.B.P.; 336355 MicroDE), and the Scottish Government Rural and Environmental Sciences and Analytical Services (RESAS) (for P.L. and S.H.D.). S.L.L.R. generated constructs and performed recombinant protein production and purification and functional characterizations of the binding protein and GHs. L.J.L., S.L., and L.M. expressed, purified, and performed functional characterization of FpCE2 and FpCE17. Growth experiments on mannans and SCFA quantifications were performed by G.L. ITC was performed by Å.K.R., Z.L., and L.S.M. G.V.P. and S.L.L.R. conducted the human metagenomic analysis. S.L.L.R., P.B.P., and B.W. conceived the study and supervised research. The manuscript was written primarily by S.L.L.R. with contributions from P.B.P., S.H.D., G.L, L.M., S.L., G.V.P., E.C.M., L.S.M., B.W., and L.J.L. Figures were prepared by S.L.L.R. We declare that we have no competing interests. Funding Information: We are grateful for support from The Research Council of Norway (FRIPRO program to P.B.P.: 250479; BIONÆR program to B.W.: 244259), the European Research Commission Starting Grant Fellowship (awarded to P.B.P.; 336355 MicroDE), and the Scottish Government Rural and Environmental Sciences and Analytical Services (RESAS) (for P.L. and S.H.D.).en
dc.description.statusPeer revieweden
dc.format.extent18
dc.format.extent4739051
dc.identifier192258373
dc.identifier85e50f17-2a2a-4b82-b8e2-38f99db230b7
dc.identifier85109132845
dc.identifier85109132845
dc.identifier34061597
dc.identifier.citationLindstad, L J, Lo, G, Leivers, S, Lu, Z, Michalak, L, Pereira, G V, Røhr, Å K, Martens, E C, McKee, L S, Louis, P, Duncan, S H, Westereng, B, Pope, P B & La Rosa, S L 2021, 'Human gut Faecalibacterium prausnitzii deploy a highly efficient conserved system to cross-feed on β-mannan-derived oligosaccharides', mBio, vol. 12, no. 3, e03628-20. https://doi.org/10.1128/mBio.03628-20en
dc.identifier.doi10.1128/mBio.03628-20
dc.identifier.iss3en
dc.identifier.issn2161-2129
dc.identifier.otherORCID: /0000-0003-2115-2399/work/99622393
dc.identifier.otherORCID: /0000-0002-4903-0978/work/99622447
dc.identifier.otherJisc: 0901449987e2488cb1226ffceca3bc0a
dc.identifier.urihttps://hdl.handle.net/2164/17036
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85109132845&partnerID=8YFLogxKen
dc.identifier.vol12en
dc.language.isoeng
dc.relation.ispartofmBioen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subjectβ-Mannanen
dc.subjectβ-Mannoligosaccharidesen
dc.subjectButyrate-produceren
dc.subjectShort-Chain Fatty Acidsen
dc.subjectCarbohydrate Active Enzymesen
dc.subjectHuman Gut Microbiotaen
dc.subjectcross-feeding interactionsen
dc.subjectR Medicineen
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subjectMicrobiologyen
dc.subjectVirologyen
dc.subjectEuropean Research Councilen
dc.subject336355en
dc.subjectRural and Environmental Science and Analytical Services (RESAS)en
dc.subjectSupplementary Dataen
dc.subject.lccRen
dc.subject.lccRA0421en
dc.titleHuman gut Faecalibacterium prausnitzii deploy a highly efficient conserved system to cross-feed on β-mannan-derived oligosaccharidesen
dc.typeJournal articleen

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