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Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells

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dc.contributor.authorKilgas, Susan
dc.contributor.authorKiltie, Anne E.
dc.contributor.authorRamadan, Kristijan
dc.contributor.institutionUniversity of Aberdeen.Rowett Instituteen
dc.date.accessioned2021-12-07T15:36:01Z
dc.date.available2021-12-07T15:36:01Z
dc.date.issued2021-12-17
dc.descriptionAcknowledgments This work was funded by the MRC Program grant MC_PC 12001/1 and MC_UU_00001/1 to K.R., and S.K. was supported by the MRC Oxford Institute of Radiation Oncology (OIRO) Cancer Research UK (CRUK) Studentship. We thank Dr. Rhodri Wilson from the microscopy imaging core (University of Oxford, Department of Oncology) for his technical advice and assistance. Graphical abstract was created with BioRender.en
dc.description.statusPeer revieweden
dc.format.extent15
dc.format.extent3939596
dc.identifier209764062
dc.identifierbbec5f92-065d-4cb5-b96d-0f1552e1ea37
dc.identifier85119911953
dc.identifier.citationKilgas, S, Kiltie, A E & Ramadan, K 2021, 'Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells', STAR Protocols, vol. 2, no. 4, 100978. https://doi.org/10.1016/j.xpro.2021.100978en
dc.identifier.doi10.1016/j.xpro.2021.100978
dc.identifier.iss4en
dc.identifier.issn2666-1667
dc.identifier.otherORCID: /0000-0001-7208-2912/work/104426466
dc.identifier.urihttps://hdl.handle.net/2164/17639
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85119911953&partnerID=8YFLogxKen
dc.identifier.vol2en
dc.language.isoeng
dc.relation.ispartofSTAR Protocolsen
dc.subjectAntibodyen
dc.subjectCell Biologyen
dc.subjectCell-based Assaysen
dc.subjectMicroscopyen
dc.subjectMolecular Biologyen
dc.subjectGeneral Biochemistry,Genetics and Molecular Biologyen
dc.subjectGeneral Neuroscienceen
dc.subjectGeneral Immunology and Microbiologyen
dc.subjectMedical Research Council (MRC)en
dc.subjectMC_PC 12001/1en
dc.subjectMC_UU_00001/1en
dc.titleImmunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cellsen
dc.typeJournal articleen

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