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Heterologous expression of a cryptic gene cluster from Streptomyces leeuwenhoekii C34T yields a novel lasso peptide, leepeptin

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dc.contributor.authorGomez-Escribano, Juan Pablo
dc.contributor.authorCastro, Jean Franco
dc.contributor.authorRazmilic, Valeria
dc.contributor.authorJarmusch, Scott A
dc.contributor.authorSaalbach, Gerhard
dc.contributor.authorEbel, Rainer
dc.contributor.authorJaspars, Marcel
dc.contributor.authorAndrews, Barbara
dc.contributor.authorAsenjo, Juan A
dc.contributor.authorBibb, Mervyn J
dc.contributor.institutionUniversity of Aberdeen.Chemistryen
dc.contributor.institutionUniversity of Aberdeen.Natural & Computing Sciencesen
dc.date.accessioned2019-11-01T14:55:01Z
dc.date.available2019-11-01T14:55:01Z
dc.date.issued2019-12-01
dc.descriptionACKNOWLEDGEMENTS. We are grateful to Michael Goodfellow and Alan Bull for providing S. leeuwenhoekii C34T , and to Michael Fischbach and Jan Claesen for S. viridochromogenes and S. pristinaspiralis, Matthias Mach for S. davawensis, and Kristian Apel on October 31, 2019 at University of Aberdeen http://aem.asm.org/ Downloaded from 17 for S. roseochromogenes. We thank Govind Chandra for advice on blastP analyses of the lasso peptide data sets, Solène Rollet for technical support in the isolation of leepeptin and Andrew Truman for his comments on the manuscript. J.F.C. and V.R. received National PhD Scholarships (#21110356 and #21110384, respectively) and Visiting Student Scholarships (Becas Chile, 2013–2014) from the National Commission for Scientific and Technological Research (CONICYT). S.A.J. thanks the University of Aberdeen for an Elphinstone Scholarship. This work was supported financially by the Biotechnological and Biological Sciences Research Council (BBSRC, United Kingdom) Institute Strategic Programme Grant “Understanding and Exploiting Plant and Microbial Secondary Metabolism” (BB/J004561/1), the Basal Programme of CONICYT (Chile) for funding of the Centre for Biotechnology and Bioengineering, CeBiB (project FB0001) and the UK Newton Project for UK–Chile collaboration (grant JIC CA586).en
dc.description.statusPeer revieweden
dc.format.extent13
dc.format.extent2929189
dc.identifier148962546
dc.identifier89c24a9f-393c-485e-af7e-fd49a427d90e
dc.identifier31562169
dc.identifier000499131000016
dc.identifier85075090863
dc.identifier.citationGomez-Escribano, J P, Castro, J F, Razmilic, V, Jarmusch, S A, Saalbach, G, Ebel, R, Jaspars, M, Andrews, B, Asenjo, J A & Bibb, M J 2019, 'Heterologous expression of a cryptic gene cluster from Streptomyces leeuwenhoekii C34 T yields a novel lasso peptide, leepeptin', Applied and Environmental Microbiology, vol. 85, no. 23, e01752-19. https://doi.org/10.1128/AEM.01752-19en
dc.identifier.doi10.1128/AEM.01752-19
dc.identifier.iss23en
dc.identifier.issn0099-2240
dc.identifier.otherORCID: /0000-0002-2426-6028/work/163664566
dc.identifier.urihttps://hdl.handle.net/2164/13206
dc.identifier.vol85en
dc.language.isoeng
dc.relation.ispartofApplied and Environmental Microbiologyen
dc.subjectStreptomycesen
dc.subjectnatural productsen
dc.subjectRiPPen
dc.subjectLasso peptidesen
dc.subjectHeterologous expressionen
dc.subjectCOELICOLORen
dc.subjectDESERTen
dc.subjectheterologous expressionen
dc.subjectRESTRICTIONen
dc.subjectBIOSYNTHESISen
dc.subjectCHAXAMYCINSen
dc.subjectSEQUENCEen
dc.subjectRESISTANCEen
dc.subjectlasso peptideen
dc.subjectBINDINGen
dc.subjectTOOLen
dc.subjectQR Microbiologyen
dc.subjectBiotechnology and Biological Sciences Research Council (BBSRC)en
dc.subjectBB/J004561/1en
dc.subject.lccQRen
dc.titleHeterologous expression of a cryptic gene cluster from Streptomyces leeuwenhoekii C34T yields a novel lasso peptide, leepeptinen
dc.typeJournal articleen

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