Isolation of highly selective IgNAR variable single-domains against a human therapeutic Fc scaffold and their application as tailor-made bioprocessing reagents

Buschhaus, Magdalena JBecker, StefanPorter, Andrew JBarelle, Caroline J2021-03-022021-03-022020-09Buschhaus, M J, Becker, S, Porter, A J & Barelle, C J 2020, 'Isolation of highly selective IgNAR variable single-domains against a human therapeutic Fc scaffold and their application as tailor-made bioprocessing reagents', Protein engineering, design & selection : PEDS, vol. 32, no. 9, pp. 385-399. https://doi.org/10.1093/protein/gzaa0021741-0126https://hdl.handle.net/2164/15951Funding This work was supported by the Industrial Biotechnology Innovation Centre, and Merck KGaA. Acknowledgements The authors would like to thank Iris Willenbücher and Kerstin Hallstein for the BIAcore™ analysis and Nadine Barron for the bio-layer interferometry work.151064309engaffinity ligandphage displaySEED bispecific antibody platformshark IgNARvariable new antigen receptorBISPECIFIC ANTIBODIESCOMPLEXIMMUNOGLOBULINANTIBODY FRAGMENTSANTIGEN RECEPTOR IGNARPLATFORMAFFINITY LIGANDSSHARKSEEDPURIFICATIONR MedicineBioengineeringMolecular BiologyBiochemistryBiotechnologyRIsolation of highly selective IgNAR variable single-domains against a human therapeutic Fc scaffold and their application as tailor-made bioprocessing reagentsJournal article10.1093/protein/gzaa002http://www.scopus.com/inward/record.url?scp=85082561719&partnerID=8YFLogxK329