Secreted inhibitors drive the loss of regeneration competence in Xenopus limbs

Aztekin, C.Hiscock, Tom W.Gurdon, JohnJullien, JeromeMarioni, John C.Simons, Benjamin David2021-06-142021-06-142021-06-30Aztekin, C, Hiscock, T W, Gurdon, J, Jullien, J, Marioni, J C & Simons, B D 2021, 'Secreted inhibitors drive the loss of regeneration competence in Xenopus limbs', Development, vol. 148, no. 11, dev199158. https://doi.org/10.1242/dev.1991580950-1991ORCID: /0000-0002-0319-8679/work/95222803https://hdl.handle.net/2164/16667ACKNOWLEDGEMENTS We thank Katarzyna Kania and the Cambridge Institute Genomics Core for their support with this work on the 10X-Genomics and sequencing library preparations. The transgenic testes used in this study was obtained from the European Xenopus Resource Centre, curated with funding from the Wellcome Trust/BBSRC, and maintained by the University of Portsmouth, School of Biological Sciences. We thank R. Jones-Green for excellent animal care. We thank H. Ma for use of her stereomicroscope, and E. Rawlins for use of her Zeiss Axiolmager compound microscope. We thank B. Steventon, E. Rawlins, and members of the Marioni and Simons labs for general discussion about the project. We thank R. Butler for assistance in image analysis. FUNDING C.A. is funded by University of Cambridge and Cambridge Trust. J.J. and J.B.G. are funded by a grant from the Wellcome Trust (101050/Z/13/Z). T.W.H., J.C.M., and B.D.S. are funded as part of a Wellcome Strategic Award to study cell fate decisions (105031/D/14/Z). T.W.H. is also supported by an EMBO Long-Term Fellowship (ALTF 606-2018). B.D.S. also acknowledges funding from the Royal Society E.P. Abraham Research Professorship (RP\R1\180165) and Wellcome Trust (098357/Z/12/Z). J.C.M. acknowledges core funding from the European Molecular Biology Laboratory and Cancer Research UK (A17197). This work is funded by a grant from the Wellcome Trust (101050/Z/13/Z), Molecular Research Council (MR/P000479/1), and supported by the Gurdon Institute core grant from Cancer Research UK (C6946/A14492) and the Wellcome Trust (092096/Z/10/Z).399160292engSDG 3 - Good Health and Well-beingLimb regenerationEx vivo limbsScRNA-SeqApical-ectodermal-ridgeXenopusR MedicineWellcome Trust101050/Z/13/Z105031/D/14/Z098357/Z/12/Z092096/Z/10/ZCancer Research UKA17197C6946/A14492OtherRP\R1\180165ALTF 606-2018Medical Research Council (MRC)MR/P000479/1Supplementary InformationRSecreted inhibitors drive the loss of regeneration competence in Xenopus limbsJournal article10.1242/dev.19915814811