Aminoacyl chain translocation catalysed by a type II thioesterase domain in an unusual non-ribosomal peptide synthetase

Wang, ShanBrittain, William D.G.Zhang, QianLu, ZhouTong, Ming HimWu, KewenKyeremeh, KwakuJenner, MatthewYu, YiCobb, Steven L.Deng, Hai2022-01-252022-01-252022-01-10Wang, S, Brittain, W D G, Zhang, Q, Lu, Z, Tong, M H, Wu, K, Kyeremeh, K, Jenner, M, Yu, Y, Cobb, S L & Deng, H 2022, 'Aminoacyl chain translocation catalysed by a type II thioesterase domain in an unusual non-ribosomal peptide synthetase', Nature Communications, vol. 13, no. 1, 62. https://doi.org/10.1038/s41467-021-27512-02041-1723ORCID: /0000-0002-0047-0622/work/159509095https://hdl.handle.net/2164/17974Funding Information: This work was supported by the Biotechnology and Biological Sciences Research Council UK (S.W. and H.D., BB/P00380X/1 and BB/R00479X/1, W.B. and S.L.C., BB/P003656/1). S.W and H.D. are grateful for SFC Covid-19 Grant extension and bridging Fund and UKRI Covid-19 Extension Allocation Fund. Q.Z. and Y.Y. are grateful for financial support from the National Key Research and Development Program of China (2018YFA0900400), the National Natural Science Foundation of China (31570033, 31811530299, and 31870035 to Y.Y.). H.D. and Y.Y. are recipients of a Royal Society-NSFC Newton Mobility Grant Award (IEC\NSFC\170617). M.J. is the recipient of a BBSRC Discovery Fellowship (BB/R012121/1). The Bruker MaXis II instrument used in this study was funded by the BBSRC (BB/M017982/1). K.K. and H.D. thank Leverhulme Trust-Royal Society Africa award (AA090088) and the jointly funded UK Medical Research Council-UK Department for International Development (MRC/DFID) Concordat agreement African Research Leaders Award (MR/S00520X/1). Publisher Copyright: © 2022, The Author(s).142052949engBiosynthesisEnzyme mechanismsEnzymesQD ChemistryQA76 Computer softwareGeneral ChemistryGeneral Biochemistry,Genetics and Molecular BiologyGeneral Physics and AstronomyBiotechnology and Biological Sciences Research Council (BBSRC)BB/P00380X/1BB/R00479X/1BB/P003656/1BB/R012121/1BB/M017982/1OtherIEC\NSFC\170617Medical Research Council (MRC)MR/S00520X/1QDQA76Aminoacyl chain translocation catalysed by a type II thioesterase domain in an unusual non-ribosomal peptide synthetaseJournal article10.1038/s41467-021-27512-0131