Balasubramanian, SandhyaAndreani, MatteoAndrade, Júlia GoncalvesSaha, TannishthaSundaravinayagam, DevakumarGarzón, JavierZhang, WenzhuPopp, OliverHiraga, Shin-IchiroRahjouei, AliRosen, Daniel BMertins, PhilippChait, Brian TDonaldson, Anne DDi Virgilio, Michela2022-04-282022-04-282022-04-13Balasubramanian, S, Andreani, M, Andrade, J G, Saha, T, Sundaravinayagam, D, Garzón, J, Zhang, W, Popp, O, Hiraga, S-I, Rahjouei, A, Rosen, D B, Mertins, P, Chait, B T, Donaldson, A D & Di Virgilio, M 2022, 'Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region', eLife, vol. 11, e75047. https://doi.org/10.7554/eLife.75047PubMedCentral: PMC9007588ORCID: /0000-0002-8722-3869/work/112495123ORCID: /0000-0002-5093-8385/work/189637779https://hdl.handle.net/2164/18493Acknowledgements We thank all members of the Di Virgilio lab for their feedback and discussion; V Delgado-Benito (Di Virgilio lab, MDC, Berlin) for her contribution to the project development; L Keller (Di Virgilio lab, MDC, Berlin) for support with cloning, mutagenesis, and mice genotyping; C Brischetto (Scheidereit Lab, MDC, Berlin) for assistance with confocal microscopy; Aberdeen Proteomics facility (University of Aberdeen) for the mass spec analysis of Aph-induced hRIF1 phosphorylation; and the MDC FACS Core Facility and Dr. HP Rahn for support with cell sorting. Aliquots of ATRi and ATMi were gener- ously provided by AG Henssen (MDC and ECRC, Berlin). Figures 1B and D, 2A, and 4C contain items created with BioRender.com. This work was supported by ERC grant 638897 (to MDV), the Helmholtz- Gemeinschaft Zukunftsthema 'Immunology and Inflammation' ZT-0027 (to MDV), P41 GM109824 and P41 GM103314 (to BTC), and Cancer Research UK awards C1445/A19059 and DRCPGM\100,013 (to ADD and SH).302366410engSDG 3 - Good Health and Well-beingAnimalsDNA/metabolismDNA Breaks, Double-StrandedDNA RepairDNA ReplicationMicePhosphorylationTelomere-Binding Proteins/geneticsR MedicineEuropean Research Council638897Cancer Research UKC1445/A19059DRCPGM\100DRCPGM\013OtherP41 GM109824P41 GM103314ZT-0027RJournal article10.7554/eLife.7504711