Harder, Christoffer BuggeMiyauchi, ShingoVirágh, MátéKuo, AlanThoen, EllaAndreopoulos, BillLu, DabaoSkrede, IngerDrula, ElodieHenrissat, BernardMorin, EmmanuelleKohler, AnnegretBarry, KerrieLaButti, KurtSalamov, AsafLipzen, AnnaMerényi, ZsoltHegedüs, BotondBaldrian, PetrStursova, MartinaWeitz, HeddaTaylor, AndyKoriabine, MaximSavage, EmilyGrigoriev, Igor V.Nagy, László G.Martin, FrancisKauserud, Håvard2024-08-202024-08-202024-07-10Harder, C B, Miyauchi, S, Virágh, M, Kuo, A, Thoen, E, Andreopoulos, B, Lu, D, Skrede, I, Drula, E, Henrissat, B, Morin, E, Kohler, A, Barry, K, LaButti, K, Salamov, A, Lipzen, A, Merényi, Z, Hegedüs, B, Baldrian, P, Stursova, M, Weitz, H, Taylor, A, Koriabine, M, Savage, E, Grigoriev, I V, Nagy, L G, Martin, F & Kauserud, H 2024, 'Extreme overall mushroom genome expansion in Mycena s.s. irrespective of plant hosts or substrate specializations', Cell Genomics, vol. 4, no. 7, 100586. https://doi.org/10.1016/j.xgen.2024.1005862666-979XORCID: /0009-0000-6758-5697/work/169079419https://hdl.handle.net/2164/24066We are highly grateful to Thomas Kehlet, Arne Aronsen, and Thomas L\u00E6ss\u00F8e for very valuable help in locating several of the Mycena specimens for culturing. We furthermore wish to thank Cecilie Mathiesen, Jacqueline Hess, and Anna Sterngren for their laboratory assistance and very useful practical suggestions. C.B.H. H.K. I.S. A.T. F.M. and L.G.N. conceived and designed the study. F.M. is coordinating the JGI CSP \u201C1KFG: Deep sequencing of ecologically-relevant Dikarya.\u201D C.B.H. and H.K. conducted fieldwork and collected new Mycena cultures. C.B.H. isolated and grew Mycena cultures, and A.T. and H.W. provided additional cultures. C.B.H. E.T. and D.L. performed DNA/RNA extraction. P.B. and M.S. analyzed the enzyme cultures. A.L. K.B. N.B. K.L. A. Kuo, and I.V.G. handled nucleic acids and sequenced, assembled, and annotated the genomes. S.M. performed the comparative secretomic, TE, and genomic analyses. A. Kuo, V.M. Z.M. and L.G.N. analyzed the genome evolution and genomic stability genes. C.B.H. V.M. Z.M. and L.G.N. performed standard/multivariate statistical tests. E.M. and A. Kuo helped analyze the TE annotation. C.B.H. L.G.N. F.M. and S.M. wrote the first version of the manuscript and crafted the revision. All authors helped finalize the manuscript and read and approved it before submission. The authors declare no competing interests. Publisher Copyright: © 2024 The Authors247416133engArctic biologybiotrophy–saprotrophy evolutioncarbon degradationfungal genomicsfungal guildgenome size diversityplant-fungus interactionsroot-associationssaprotrophsTE proliferationQH301 BiologyBiochemistry, Genetics and Molecular Biology (miscellaneous)GeneticsSupplementary Informationhttps://doi.org/10.1016/j.xgen.2024.100586QH301Journal article10.1016/j.xgen.2024.100586http://www.scopus.com/inward/record.url?scp=85198033926&partnerID=8YFLogxK47