Kilgas, SusanKiltie, Anne E.Ramadan, Kristijan2021-12-072021-12-072021-12-17Kilgas, S, Kiltie, A E & Ramadan, K 2021, 'Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells', STAR Protocols, vol. 2, no. 4, 100978. https://doi.org/10.1016/j.xpro.2021.1009782666-1667ORCID: /0000-0001-7208-2912/work/104426466https://hdl.handle.net/2164/17639Acknowledgments This work was funded by the MRC Program grant MC_PC 12001/1 and MC_UU_00001/1 to K.R., and S.K. was supported by the MRC Oxford Institute of Radiation Oncology (OIRO) Cancer Research UK (CRUK) Studentship. We thank Dr. Rhodri Wilson from the microscopy imaging core (University of Oxford, Department of Oncology) for his technical advice and assistance. Graphical abstract was created with BioRender.153939596engAntibodyCell BiologyCell-based AssaysMicroscopyMolecular BiologyGeneral Biochemistry,Genetics and Molecular BiologyGeneral NeuroscienceGeneral Immunology and MicrobiologyMedical Research Council (MRC)MC_PC 12001/1MC_UU_00001/1Journal article10.1016/j.xpro.2021.100978http://www.scopus.com/inward/record.url?scp=85119911953&partnerID=8YFLogxK24