Redox Regulation, Rather than Stress-Induced Phosphorylation, of a Hog1 Mitogen-Activated Protein Kinase Modulates Its Nitrosative-Stress-Specific Outputs

Herrero de Dios, CarmenDay, Alison MTillmann, Anna TKastora, Stavroula LStead, DavidSalgado, Paula S.Quinn, JanetBrown, Alistair J P2018-03-272018-03-272018-03-27Herrero de Dios, C, Day, A M, Tillmann, A T, Kastora, S L, Stead, D, Salgado, P S, Quinn, J & Brown, A J P 2018, 'Redox Regulation, Rather than Stress-Induced Phosphorylation, of a Hog1 Mitogen-Activated Protein Kinase Modulates Its Nitrosative-Stress-Specific Outputs', mBio, vol. 9, no. 2, e02229-17. https://doi.org/10.1128/mBio.02229-17ORCID: /0000-0001-9268-5868/work/76974346http://hdl.handle.net/2164/10244Data availability. The RNA sequencing dataset is available at EBI (www.ebi.ac.uk/arrayexpress/) under accession number E-MTAB-5990. Other data that support the findings of this study are available from the corresponding author upon reasonable request. ACKNOWLEDGMENTS We thank Debbie Smith for constructing the strains JC41 and JC310, Arnab Pradhan for help with DHE control experiments, and our colleagues in the Aberdeen Fungal Group and Newcastle Yeast Group for insightful discussions. We are also grateful to Mike Gustin for his advice. We are grateful to the Centre for Genome Enabled Biology and Medicine, Aberdeen Proteomics, the Iain Fraser Cytometry Centre, the Microscopy and Histology Facility, and the qPCR facility at the University of Aberdeen for their help, advice, and support. This work was funded by the UK Biotechnology and Biological Research Council (http://www.bbsrc.ac.uk) (grants BB/K017365/1 and BB/F00513X/1 to A.J.P.B. and grant BB/K016393/1 to J.Q.). This work was also supported by the European Research Council (http://erc.europa.eu/) (STRIFE advanced grant C-2009-AdG-249793 to A.J.P.B.), the UK Medical Research Council (http://www.mrc.ac.uk) (grant MR/M026663/1 to A.J.P.B. and grant MR/M000923/1 to P.S.S.), the Wellcome Trust (https://wellcome.ac.uk) (grant 097377 to A.J.P.B. and J.Q.), the MRC Centre for Medical Mycology and the University of Aberdeen (grant MR/M026663/1 to A.J.P.B.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.162132429engMAP kinase signallingHog1post-translational modificationnitrosative stresstranscript profilingCandida albicansR MedicineBiotechnology and Biological Sciences Research Council (BBSRC)BB/K017365/1BB/F00513X/1BB/K016393/1Medical Research Council (MRC)MR/M026663/1MR/M000923/1Wellcome Trust097377European Research CouncilC-2009-AdG-249793Supplementary DataRRedox Regulation, Rather than Stress-Induced Phosphorylation, of a Hog1 Mitogen-Activated Protein Kinase Modulates Its Nitrosative-Stress-Specific OutputsJournal article10.1128/mBio.02229-1792