Yamamoto, TakayoshiKambayashi, YutaOtsuka, YutaAfouda, BoniGiuraniuc, VasileMichiue, TatsuoHoppler, Stefan2022-08-152022-08-152022-08-09Yamamoto, T, Kambayashi, Y, Otsuka, Y, Afouda, B, Giuraniuc, V, Michiue, T & Hoppler, S 2022, 'Positive feedback regulation of fzd7 expression robustly shapes a steep Wnt gradient in Xenopus heart development, together with sFRP1 and heparan sulfate', eLife, vol. 11, 73818. https://doi.org/10.7554/eLife.73818https://hdl.handle.net/2164/19064Acknowledgements We thank Dr. Yukio Nakamura (Aberdeen University, UK; Present address: Repertoire Genesis Inc., Japan.) and Dr. Masanori Taira (Chuo University, Japan) for their help in initiating this project; Dr. Takehiko Nakamura (Seikagaku Corporation, Japan) for NAH46 antibody and hybridoma; Dr. Osamu Yoshie (Kindai University, Japan) for HepSS-1 hybridoma; Dr. Makoto Matsuyama (Shigei Medical Research Institute, Japan) for the contribution to the generation of NAH46 and HepSS-1 antibody from the hybridomas; Dr. Steven D. Aird for technical editing of the manuscript. This international collaboration was supported in part by Daiwa Anglo-Japanese Foundation (12969/13787 to T.Y., B.A., T.M., and S.H.); with additional research support in Japan from MEXT/JSPS KAKENHI (19K16138 to T.Y., 18K06244/21K06183 to T.Y. and T.M.); and in the United Kingdom from BHF (RG/18/8/33673 to S.H.) and BBSRC (BB/N021924/1; BB/M001695/1 to S.H.). S.H. was a Royal Society/Leverhulme Trust Senior Research Fellow (SRF\R1\191017).216313811engWnt signalFrizzledHeartGene Regulatory CircuitMorphogenxenopusR MedicineBiotechnology and Biological Sciences Research Council (BBSRC)BB/N021924/1BB/M001695/1OtherSRF\R1\191017Supplementary DataRJournal article10.7554/eLife.7381811