An analysis of possible off target effects following CAS9/CRISPR targeted deletions of neuropeptide gene enhancers from the mouse genome

Hay, Elizabeth AnneKhalaf, Abdulla RazakMarini, PietroBrown, AndrewHeath, KarynSheppard, DarrinMacKenzie, Alasdair2016-11-302016-11-302017-08Hay, E A, Khalaf, A R, Marini, P, Brown, A, Heath, K, Sheppard, D & MacKenzie, A 2017, 'An analysis of possible off target effects following CAS9/CRISPR targeted deletions of neuropeptide gene enhancers from the mouse genome', Neuropeptides, vol. 64, pp. 101-107. https://doi.org/10.1016/j.npep.2016.11.0030143-4179ORCID: /0000-0002-8456-4473/work/177758657http://hdl.handle.net/2164/7773Acknowledgements These studies were funded by a Wellcome Trust (105625/Z/14/Z) ISSF starting grant, Medical Research Scotland (PhD-719-2013) and GW Pharmaceuticals (PhD-719-2013 - S.5242.001).71487184engneuropeptideenhancerCAS9/CRISPROff-target effectstransgenic mouseCAS9 mRNAgRNA1-cell mouse embryoCytoplasmic microinjectionCRISPR design toolUCSC browser BLATPCRSanger sequencingR MedicineWellcome Trust105625/Z/14/ZRAn analysis of possible off target effects following CAS9/CRISPR targeted deletions of neuropeptide gene enhancers from the mouse genomeJournal article10.1016/j.npep.2016.11.00364