van den Berg, JonasGalbiati, HeloisaRasmussen, AkikoMiller, SamanthaPoolman, Bert2016-09-062016-09-062016-09-06van den Berg, J, Galbiati, H, Rasmussen, A, Miller, S & Poolman, B 2016, 'On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli', Scientific Reports, vol. 6, 32709. https://doi.org/10.1038/srep327092045-2322ORCID: /0000-0002-8223-9693/work/76974586http://hdl.handle.net/2164/7449We thank Frans Bianchi and Franz Ho for assistance with molecular cloning, Tim Rasmussen for providing the pTRC-MscK plasmid, Andrew Robinson for providing the pBAD-mEos3.2 plasmid, Matthias Heinemann for assistance with the flow cytometry measurements, Paul Schavemaker for performing Smoldyn simulations and Michiel Punter for programming ImageJ plugins for PALM reconstructions and single-particle tracking. We thank Ian Booth for critical reading of the manuscript, and Christoffer Åberg and Matteo Gabba for valuable discussions. The authors would like to thank David Dryden and Marcel Reuter for performing preliminary experiments from which this work has been built. The work was funded by the EU FP7 ITN-network program NICHE.1158041engmechanosensitive channelsosmoregulationsuper-resolution microscopylateral diffusionquantitative biologysingle-molecule analysisR MedicineRJournal article10.1038/srep327096