Rasmussen, TimRasmussen, AkikoSingh, ShivaniGalbiati, HeloisaEdwards, Michelle D.Miller, SamanthaBooth, Ian R.2015-07-302015-07-302015-07Rasmussen, T, Rasmussen, A, Singh, S, Galbiati, H, Edwards, M D, Miller, S & Booth, I R 2015, 'Properties of the mechanosensitive channel MscS pore revealed by tryptophan scanning mutagenesis', Biochemistry, vol. 54, no. 29, pp. 4519-4530. https://doi.org/10.1021/acs.biochem.5b002940006-2960ORCID: /0000-0002-8223-9693/work/76974588http://hdl.handle.net/2164/4729Funding This work was supported by a Wellcome Trust Programme grant [092552/A/10/Z awarded to I.R.B., S.M., J. H. Naismith (University of St Andrews, St Andrews, U.K.), and S. J. Conway (University of Oxford, Oxford, U.K.)] (T.R. and M.D.E.), by a BBSRC grant (A.R.) [BB/H017917/1 awarded to I.R.B., J. H. Naismith, and O. Schiemann (University of St Andrews)], by a Leverhulme Emeritus Fellowship (EM-2012-060\2), and by a CEMI grant to I.R.B. from the California Institute of Technology. The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013 FP7/2007-2011) under Grant PITN-GA-2011-289384 (FP7-PEOPLE-2011-ITN NICHE) (H.G.) (awarded to S.M.).123966005engfluorescencebacterial homeostasishypoosmotic shockcomplex stabilitymembrane proteinpore hydrationQ ScienceWellcome Trust092552/A/10/ZBiotechnology and Biological Sciences Research Council (BBSRC)BB/H017917/1 aQJournal article10.1021/acs.biochem.5b002945429