A nematode-specific ribonucleoprotein complex mediates interactions between the major nematode spliced leader snRNP and its target pre-mRNAs

Eijlers, PeterAl-Khafaji, Mohammed IsmaelSoto Martin, EvaFasimoye, Rotimi YemiStead, DavidWenzel, MariusMuller, BerndtPettitt, Jonathan2024-06-182024-06-182024-07-08Eijlers, P, Al-Khafaji, M I, Soto Martin, E, Fasimoye, R Y, Stead, D, Wenzel, M, Muller, B & Pettitt, J 2024, 'A nematode-specific ribonucleoprotein complex mediates interactions between the major nematode spliced leader snRNP and its target pre-mRNAs', Nucleic Acids Research, vol. 52, no. 12, gkae321, pp. 7245-7260. https://doi.org/10.1093/nar/gkae3210305-1048ORCID: /0000-0003-1279-5421/work/159077956ORCID: /0000-0001-9268-5868/work/159077960ORCID: /0000-0003-0093-368X/work/159078016ORCID: /0000-0001-7988-6833/work/160389546ORCID: /0009-0009-3364-1174/work/188340731https://hdl.handle.net/2164/23650Acknowledgements We thank Maheshika Kurukulasuriya for her assistance with immunoprecipitations from embryo extracts. Some strains were provided by the Caenorhabditis Genetics Center, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Sequencing was performed by the Centre for Genome-Enabled Biology and Medicine of the University of Aberdeen, and proteomics analysis by the Aberdeen proteomics facility. We thank Kate Burgoyne and Craig Pattinson (Aberdeen Proteomics) for technical support. We thank WormBase for providing the community resource that facilitated the interrogation of C. elegans molecular genetics used in this work . Author contribution: P.E., M.A., E.S.-M., R.F., M.W., B.M. and J.P. contributed experiments. J.P. and B.M. conceived the research and managed and coordinated the research activity; J.P. and B.M. acquired funding for the project; D.S. guided the proteomics analysis, M.W., B.M. and J.P. designed and implemented the computational analysis; M.W., B.M. and J.P. wrote the manuscript and prepared figures and tables.162192019engAnimalsRNA, Spliced Leader/metabolismRNA Precursors/metabolismRibonucleoproteins, Small Nuclear/metabolismSpliceosomes/metabolismCaenorhabditis elegans/geneticsCaenorhabditis elegans Proteins/metabolismTrans-SplicingProtein BindingR MedicineBiotechnology and Biological Sciences Research Council (BBSRC)BB/T002859/1Supplementary Datahttps://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkae321#supplementary-dataRA nematode-specific ribonucleoprotein complex mediates interactions between the major nematode spliced leader snRNP and its target pre-mRNAsJournal article10.1093/nar/gkae321https://abdn.elsevierpure.com/en/publications/e59362a0-614f-44fe-ad5f-48014c57ed365212