Cuthbertson, LeahRogers, Geraint BWalker, Alan WOliver, AnnaHoffman, Lucas RCarroll, Mary PParkhill, JulianBruce, Kenneth Dvan der Gast, Christopher J2015-06-292015-06-292015-07Cuthbertson, L, Rogers, G B, Walker, A W, Oliver, A, Hoffman, L R, Carroll, M P, Parkhill, J, Bruce, K D & van der Gast, C J 2015, 'Implications of multiple freeze-thawing on respiratory samples for culture-independent analyses', Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, vol. 14, no. 4, pp. 464-467. https://doi.org/10.1016/j.jcf.2014.10.0041873-5010ORCID: /0000-0001-5099-8495/work/42316325http://hdl.handle.net/2164/4657Copyright © 2014. Published by Elsevier B.V. Acknowledgements This study was supported by the UK Natural Environment Research Council (NE/H019456/1) to CJvdG, by the Wellcome Trust (WT 098051) to AWW and JP, and by the US National Institute of Health (K02HL105543 and P30 DK089507) to LRH.4315424engmicrobiotamicrobiomebiobankpyrosequencingpropidium monoazidesputumR MedicineNatural Environment Research Council (NERC)RJournal article10.1016/j.jcf.2014.10.004144